ValidPrime Validprime kit:ValidPrime® Mouse – SYBR assay – 250 rxns The goal of the analysis

The goal of the analysis is to find the optimum concentration for the NGS library

TATAA Probe GrandMaster® Mix (2x) (1 tube á 1250 µl)

study-specific housekeeping gene

which detect the presence of both alleles in a single test tube

c endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM

ValidPrime Validprime kit:ValidPrime® Mouse – SYBR assay – 250 rxns The goal of the analysisValidPrime replaces the need to perform no reverse transcriptase (RT( )) controls to test for the presence of genomic DNA (gDNA) for all samples in your real time quantitative PCR (qPCR) profiling. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT( ) reactions plus m x n qPCR controls. When using ValidPrime only m + n + 1 controls are needed. ValidPrime will minimize the amount of control reactions